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The more constraints that are available from NMR data, the more accurate the resulting energy minimised structure will be. Both short and long range NOE data are interpolated by energy minimisation calculations as through-space constraints in order to derive a nal protein structure. The other main types of constraint in use are angular constraints, deriving from 3 1 ] H 1 H homonuclear and 3] 1 H 15 N heteronuclear constant data, and distance constraints that derive from chemical shift data, which demonstrates the existence of hydrogen bonds and residual dipolar coupling. Energy minimisation is beyond the scope of this book (at this stage at least!) so will not be described further except to say that the process can be imprecise and the number of long range distance constraints heavily in uences the accuracy of the structural output. In consideration of this, NMR energy minimised structures are usually presented in toto in the form of an ensemble or family of different minimised structures that derive from the same basis set of short and long range NOE data. The average of this family usually compares very closely with the protein structure when available as determined by X-ray crystallography (Figure 5.28). Any major differences between derived structures from the two techniques are generally the result of dynamic regions within a protein structure that are typically frozen in place within a crystal environment.

3 0.0000000107410370 0.0000000036356241 0.0000000030740142 0.0000000064434525 0.0000000065792000 0.0000000059127633 0.0000000017515015 0.0000000073567052 0.0000000075741396 0.0000000036721247 0.0000000057486475 0.0000000034975604 0.0000000089735489

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, Academic Press, London, 1982, 2, p 44 142 W R Moore and H R Ward, J Am Chem Soc 85, 86 (1963) 143 T J Jacobs and R Akawie, R G Cooper, J Am Chem Soc 73, 1273 (1951) 144 W Ziegenbein and W M Schneider, Chem Ber 98, 824 (1965) 145 T L Jacobs and D Dankner, J Org Chem 22, 1424 (1957) 146 T J Jacobs, D Dankner, and S Singer, Tetrahedron 20, 2177 (1964) 147 M D Carr, L H Gan, and 1 Reid, J Chem Soc, Perkin Trans 2 668, 672 (1973) 148 D J Cram, F Willey, H P Fischer, H M Relles, and D A Scott, J Am Chem Soc 88, 2759 (1966) 149 J Klein and S Brenner, J Chem Soc, Chem Commun 1020 (1969) 150 B A Kasansky and A F Plate, Chem Ber 69, 1862 (1936) 151 J.

Characteristics of the link layer of wireless networks (i.e. 2.5G/3G) have significant effects on TCP and application layers performance. Big latencies, variable data rates, asymmetry, delay spikes, data loss and bandwidth oscillations are some of the main effects that can affect to the performance of TCP [34]. For this reason, it is important to understand how these different effects interact with the protocol, in order to find out possible improvements or parameter optimizations that could help to reduce any degradation. A summary of those effects is described below (further details can be found in section 5.4.1.1).

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R Anderson, Adv Catal 23, 1 (1973) 152 J K A Clarke and J J Rooney, Adv Catal25, 125 (1976) 153 V Amir-Ebrahimi, F Garin, F Weisang, and F G Gault, New J Chem 3, 529 (1979) 154 F G Gault, Adv Catal 30, 1 (1981) 155 J R Anderson and N R Avery, J Catal 5,446 (1966); 7, 315 (1967) 156 J M Muller and F G Gault, Proc 4th/nt Congr Catal, Moscow, 1968; Symposium on Kinetics and Mechanism of Complex Catalytic Reactions, Paper 15 157 M A McKervey, J J Rooney, and N G Samman, J Catal 30, 330 (1973) 158 F Garin and F G Gault, J Am Chem Soc 97, 4466 (1975) 159 F G Gault, J J Rooney, and C Kemball, J Catal 1,255 (1962) 160 Y Barron, G Maire, J M Muller, and F G Gault, J Catal 5, 428 (1966) 161.

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obtaining necessary authentication credentials (connectivity layer protocols); querying an information system and replica catalog to determine availability of computers, storage systems, and networks, and the location of required input les (collective services); submitting requests to appropriate computers, storage systems, and networks to initiate computations, move data, and so forth (resource protocols); and monitoring the progress of the various computations and data transfers, notifying the user when all are completed, and detecting and responding to failure conditions (resource protocols).

Type Research Research Research Research Research Research Research Working Area Working Area Area Area Area